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KMID : 0385020030190010029
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Korean Journal of Laboratory Animal Science
2003 Volume.19 No. 1 p.29 ~ p.33
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Development of Inherited Dominant Tail Deformity Mouse Induced by ENU-Mutagenesis
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김양언/Kim, Yang-Eon
조재우/조규혁/한상섭/송창우/Cho, Jae-Woo/Cho, Kyu-Hyuk/Han, Sang-Seop/Song, Chang-Woo
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Abstract
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ENU(N-ethyl-N-nitrosourea) is a powerful germline mutagen in the mouse. It has been used to develop a new or allelic mutant mouse as disease model animals. We injected fractionated dose of 200 mg/kg ENU into male BALB/c (G_0) intraperitoneally and selected several kinds of mice with abnormal phenotypes in the firs generation (G_1). Among them, two mutant founders (G_1) showing bent, kinky or circled tail were identified. One was male (ADM22) and the other was female (ADF16). These mice exhibited no other structural anomaly except tail deformity. G_1 (+/-) mice were mated to normal (+/+) and the tail deformity mice were found in the G_2. Tail anomaly of mutants was visible and distinguished from normal littermates at birth. The mutant incidence was 40% (ADM22) and 41% (ADF16) in backcross and 72% (ADM22) and 71% (ADF16) in intercross. The sex ration was close to 1:1 (p>0.05). It was thought that this mutation gene was inherited by single autosomal dominant trait.
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